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Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells.
DC Field | Value | Language |
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dc.contributor.author | Kim, J | - |
dc.contributor.author | Kim, HN | - |
dc.contributor.author | Lim, KT | - |
dc.contributor.author | Kim, Y | - |
dc.contributor.author | Pandey, S | - |
dc.contributor.author | Garg, P | - |
dc.contributor.author | Choung, YH | - |
dc.contributor.author | Choung, PH | - |
dc.contributor.author | Suh, KY | - |
dc.contributor.author | Chung, JH | - |
dc.date.accessioned | 2014-05-29T02:30:06Z | - |
dc.date.available | 2014-05-29T02:30:06Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/10266 | - |
dc.description.abstract | Inspired by the aligned nanostructures and co-existence of vascular cells and stem cells in human cancellous bone, we quantitatively investigated the relative contributions of nanotopography and co-culture with human umbilical endothelial cells (HUVECs) to the osteogenesis of human mesenchymal stem cells (hMSCs). Although both nanotopography and co-culture independently enhanced the osteogenesis of hMSCs, osteogenesis was further enhanced by the two factors in combination, indicating the importance of synergistic cues in stem cell engineering. Interestingly, nanotopography provided a larger relative contribution to the osteogenesis of hMSCs than did co-culture with HUVECs. Furthermore, the osteogenesis of hMSCs was also affected by the density of parallel nanogrooves, exhibiting a maximum at a 1:3 spacing ratio, as defined as the ratio of ridge width to groove width. Analysis of (i) biochemical soluble factors, (ii) hMSC-substrate interaction and (iii) hMSC-HUVEC interaction suggests that (ii) and (iii) play a crucial role in mediating osteogenic phenotypes. | - |
dc.language.iso | en | - |
dc.subject.MESH | Bone and Bones | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Shape | - |
dc.subject.MESH | Cellular Microenvironment | - |
dc.subject.MESH | Coculture Techniques | - |
dc.subject.MESH | Human Umbilical Vein Endothelial Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mesenchymal Stromal Cells | - |
dc.subject.MESH | Nanoparticles | - |
dc.subject.MESH | Osteogenesis | - |
dc.subject.MESH | Time Factors | - |
dc.title | Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells. | - |
dc.type | Article | - |
dc.identifier.pmid | 23834896 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0142-9612(13)00715-1 | - |
dc.contributor.affiliatedAuthor | 정, 연훈 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.biomaterials.2013.06.029 | - |
dc.citation.title | Biomaterials | - |
dc.citation.volume | 34 | - |
dc.citation.number | 30 | - |
dc.citation.date | 2013 | - |
dc.citation.startPage | 7257 | - |
dc.citation.endPage | 7268 | - |
dc.identifier.bibliographicCitation | Biomaterials, 34(30). : 7257-7268, 2013 | - |
dc.identifier.eissn | 1878-5905 | - |
dc.relation.journalid | J001429612 | - |
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