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Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells.

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dc.contributor.authorKim, J-
dc.contributor.authorKim, HN-
dc.contributor.authorLim, KT-
dc.contributor.authorKim, Y-
dc.contributor.authorPandey, S-
dc.contributor.authorGarg, P-
dc.contributor.authorChoung, YH-
dc.contributor.authorChoung, PH-
dc.contributor.authorSuh, KY-
dc.contributor.authorChung, JH-
dc.date.accessioned2014-05-29T02:30:06Z-
dc.date.available2014-05-29T02:30:06Z-
dc.date.issued2013-
dc.identifier.issn0142-9612-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/10266-
dc.description.abstractInspired by the aligned nanostructures and co-existence of vascular cells and stem cells in human cancellous bone, we quantitatively investigated the relative contributions of nanotopography and co-culture with human umbilical endothelial cells (HUVECs) to the osteogenesis of human mesenchymal stem cells (hMSCs). Although both nanotopography and co-culture independently enhanced the osteogenesis of hMSCs, osteogenesis was further enhanced by the two factors in combination, indicating the importance of synergistic cues in stem cell engineering. Interestingly, nanotopography provided a larger relative contribution to the osteogenesis of hMSCs than did co-culture with HUVECs. Furthermore, the osteogenesis of hMSCs was also affected by the density of parallel nanogrooves, exhibiting a maximum at a 1:3 spacing ratio, as defined as the ratio of ridge width to groove width. Analysis of (i) biochemical soluble factors, (ii) hMSC-substrate interaction and (iii) hMSC-HUVEC interaction suggests that (ii) and (iii) play a crucial role in mediating osteogenic phenotypes.-
dc.language.isoen-
dc.subject.MESHBone and Bones-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCell Shape-
dc.subject.MESHCellular Microenvironment-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHHuman Umbilical Vein Endothelial Cells-
dc.subject.MESHHumans-
dc.subject.MESHMesenchymal Stromal Cells-
dc.subject.MESHNanoparticles-
dc.subject.MESHOsteogenesis-
dc.subject.MESHTime Factors-
dc.titleSynergistic effects of nanotopography and co-culture with endothelial cells on osteogenesis of mesenchymal stem cells.-
dc.typeArticle-
dc.identifier.pmid23834896-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0142-9612(13)00715-1-
dc.contributor.affiliatedAuthor정, 연훈-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.biomaterials.2013.06.029-
dc.citation.titleBiomaterials-
dc.citation.volume34-
dc.citation.number30-
dc.citation.date2013-
dc.citation.startPage7257-
dc.citation.endPage7268-
dc.identifier.bibliographicCitationBiomaterials, 34(30). : 7257-7268, 2013-
dc.identifier.eissn1878-5905-
dc.relation.journalidJ001429612-
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Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
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