Cited 0 times in
In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Cho, SH, | - |
dc.contributor.author | Chang, WS | - |
dc.contributor.author | Kim, JG | - |
dc.contributor.author | Whang, KH | - |
dc.contributor.author | Choi, KS | - |
dc.contributor.author | Sohn, SH | - |
dc.date.accessioned | 2011-01-14T01:34:03Z | - |
dc.date.available | 2011-01-14T01:34:03Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0169-4332 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/1135 | - |
dc.description.abstract | The photo-bleaching of single living cells excited by femtosecond laser irradiation was observed in situ to study the nonlinear interaction between ultrafast laser pulses and living human breast MDA-MB-231 cells. We conducted a systematic study of the energy dependence of plasma-mediated photo-disruption of fluorescently labeled subcellular structures in the nucleus of living cells using near-infrared (NIR) femtosecond laser pulses through a numerical aperture objective lens (0.75 NA). The behavior of photo-bleached living cells with fluorescently labeled nuclei was observed for 18 h after femtosecond laser irradiation under a fluorescence microscope. The photo-bleaching of single living cells without cell disruption occurred at between 470 and 630 nJ. To study the photo-disruption of subcellular organelles in single living cells using the nonlinear absorption excited by a NIR femtosecond laser pulse, the process of photo-bleaching without photo-disruption provides key information for clarifying the nonlinear interaction between NIR ultrashort, high-intensity laser light and transparent fluorescently labeled living cells. | - |
dc.format | application/pdf | - |
dc.language.iso | en | - |
dc.title | In situ observation of photo-bleaching in human single living cell excited by a NIR femtosecond laser. | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | 최, 경숙 | - |
dc.contributor.affiliatedAuthor | 손, 성향 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.apsusc.2007.11.030 | - |
dc.citation.title | Applied surface science | - |
dc.citation.volume | 254 | - |
dc.citation.number | 11 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 3370 | - |
dc.citation.endPage | 3375 | - |
dc.identifier.bibliographicCitation | Applied surface science, 254(11). : 3370-3375, 2008 | - |
dc.relation.journalid | J001694332 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.