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Gas chromatographic-mass spectrometric analyses of cholesterol and its precursors in rat plasma as tert-butyldimethylsilyl derivatives.

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dc.contributor.authorPaik, MJ-
dc.contributor.authorYu, J-
dc.contributor.authorHu, MB-
dc.contributor.authorKim, SJ-
dc.contributor.authorKim, KR-
dc.contributor.authorAhn, YH-
dc.contributor.authorChoi, S-
dc.contributor.authorLee, G-
dc.date.accessioned2011-01-14T05:18:40Z-
dc.date.available2011-01-14T05:18:40Z-
dc.date.issued2008-
dc.identifier.issn0009-8981-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1151-
dc.description.abstractBACKGROUND: Cholesterol and its metabolic precursors occurring in metabolic pathways are important biochemical indicators in pathological conditions.



METHODS: A method for the simultaneous determination of cholesterol and its metabolic precursors, such as lanosterol and 7-dehydrocholesterol, in rat plasma is demonstrated. It involves their extraction after saponification, followed by conversion to tert-butyldimethylsilyl (TBDMS) derivatives for analysis by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode (GC-SIM-MS).



RESULTS: The characteristic fragment ions of [M-57], m/z 443, 483, and 441 permitted the accurate and selective detection of cholesterol and its precursors in rat plasma. The whole procedure of TBDMS derivatization, with subsequent GC-SIM-MS analysis, was linear (r>or=0.9994), reproducible (% relative standard deviation=2.2 to 7.5), and accurate (% relative error=-5.6 to 7.7), with detection limits of 0.02 to 0.07 ng/ml. Recoveries were measured to be ranged from 89.5 to 95.4%.



CONCLUSION: The present method was useful for the quantification of cholesterol and its precursors in rat plasma samples of 1 microl.
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dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHCholesterol-
dc.subject.MESHGas Chromatography-Mass Spectrometry-
dc.subject.MESHMale-
dc.subject.MESHMethylation-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHSpectrometry, Mass, Electrospray Ionization-
dc.titleGas chromatographic-mass spectrometric analyses of cholesterol and its precursors in rat plasma as tert-butyldimethylsilyl derivatives.-
dc.typeArticle-
dc.identifier.pmid18657530-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0009-8981(08)00328-8-
dc.contributor.affiliatedAuthor백, 만정-
dc.contributor.affiliatedAuthor안, 영환-
dc.contributor.affiliatedAuthor이, 광-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.cca.2008.06.025-
dc.citation.titleClinica chimica acta; international journal of clinical chemistry-
dc.citation.volume396-
dc.citation.number1-2-
dc.citation.date2008-
dc.citation.startPage62-
dc.citation.endPage65-
dc.identifier.bibliographicCitationClinica chimica acta; international journal of clinical chemistry, 396(1-2). : 62-65, 2008-
dc.identifier.eissn1873-3492-
dc.relation.journalidJ000098981-
Appears in Collections:
Journal Papers > Research Organization > Institute for Neuroregeneration & Stem Cell Research
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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