Cited 0 times in Scipus Cited Count

Optimization of critical factors affecting the performance of an allergen chip for the analysis of an allergen-specific human IgE in serum.

DC Field Value Language
dc.contributor.authorHan, MK-
dc.contributor.authorSeo, MH-
dc.contributor.authorLee, D-
dc.contributor.authorKim, SH-
dc.contributor.authorPark, HS-
dc.contributor.authorKim, HS-
dc.date.accessioned2011-01-26T01:55:05Z-
dc.date.available2011-01-26T01:55:05Z-
dc.date.issued2007-
dc.identifier.issn0910-6340-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1275-
dc.description.abstractA sensitive and multiplexed assay of allergen-specific human immunoglobulin E (IgE) is of great significance in the precise diagnosis of allergies. We report on the optimization of critical factors for chip-based analysis of IgE in human serum with a high reliability. Extracts of two mite species were used as model allergens, and were spotted onto a glass slide for the construction of an allergen chip. Respective allergen-specific IgE in human serum was analyzed by using biotinylated anti-human IgE and a streptavidin-Cy3 conjugate. Factors affecting the performance of the allergen chip were investigated and optimized. Especially, the effect of additives, the concentrations of biotinylated anti-human IgE and the streptavidin-Cy3 conjugate, the serum dilution factor, and the concentration of allergen extract as a capturing agent were examined in detail. Under the optimized conditions, a chip-based analysis for sera from 43 patients revealed a reliable and reproducible diagnosis of respective allergies, showing a good correlation with a conventional CAP assay.-
dc.language.isoen-
dc.subject.MESHAllergens-
dc.subject.MESHAnimals-
dc.subject.MESHAntibody Specificity-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHHumans-
dc.subject.MESHImmunoglobulin E-
dc.subject.MESHProtein Array Analysis-
dc.subject.MESHPyroglyphidae-
dc.subject.MESHSensitivity and Specificity-
dc.subject.MESHSpecies Specificity-
dc.titleOptimization of critical factors affecting the performance of an allergen chip for the analysis of an allergen-specific human IgE in serum.-
dc.typeArticle-
dc.identifier.pmid17495399-
dc.identifier.urlhttp://joi.jlc.jst.go.jp/JST.JSTAGE/analsci/23.545?from=PubMed-
dc.contributor.affiliatedAuthor김, 승현-
dc.contributor.affiliatedAuthor박, 해심-
dc.type.localJournal Papers-
dc.citation.titleAnalytical sciences-
dc.citation.volume23-
dc.citation.number5-
dc.citation.date2007-
dc.citation.startPage545-
dc.citation.endPage549-
dc.identifier.bibliographicCitationAnalytical sciences, 23(5). : 545-549, 2007-
dc.identifier.eissn1348-2246-
dc.relation.journalidJ009106340-
Appears in Collections:
Journal Papers > Hospital > Clinical Trial Center
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Files in This Item:
There are no files associated with this item.

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse