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Effects of platelet lysate preparations on the proliferation of HaCaT cells.

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dc.contributor.authorBaik, SY-
dc.contributor.authorLim, YA-
dc.contributor.authorKang, SJ-
dc.contributor.authorAhn, SSSH-
dc.contributor.authorLee, WG-
dc.contributor.authorKim, CH-
dc.date.accessioned2016-11-18-
dc.date.available2016-11-18-
dc.date.issued2014-
dc.identifier.issn2234-3806-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/12893-
dc.description.abstractBACKGROUND: Standard protocols are lacking for the preparation of platelet

lysates (PL) as an alternative to using fetal bovine serum as a cell culture

supplement. This study aimed to establish optimum conditions for preparing PL for

use in cell cultures. METHODS: Cell density in three pooled platelet concentrates

(PC) were adjusted to 1x10(12)/L and 2x10(12)/L. PL was prepared from PC by 1 to

3 freeze-thaw (FT) cycles. HaCaT cells were cultured in media supplemented with

5% or 10% PL. Cell numbers were estimated using a Cell Counting Kit-8 (CCK-8;

Dojindo Laboratories, Japan). Growth factors were quantified by using the Luminex

200 system (Luminex Corporation, USA). RESULTS: Cell proliferation rates in the

presence of PLs were similar when prepared from PCs of both cell densities. The

rates were higher in media containing 5% PL than 10% PL when prepared by two FT

cycles. Concentrations of vascular endothelial growth factor (VEGF),

platelet-derived growth factor-AB/BB (PDGF-AB/BB), PDGF-AA, and epidermal growth

factor (EGF) were significantly higher in PL prepared from PC with a cell density

of 2x10(12)/L than 1x10(12)/L PC. However, only VEGF and PDGF-AA concentrations

in PLs were correlated with HaCaT cell counts. CONCLUSIONS: The 5% PL from PC

with a cell density of 1x10(12)/L prepared by two FT cycles treatment was the

most effective condition that supported steady HaCaT cell proliferation. Our

finding may be useful for preparing PL-supplemented cell culture media.
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dc.language.isoen-
dc.subject.MESHBlood Platelets-
dc.subject.MESHCell Line-
dc.subject.MESHCell Proliferation-
dc.subject.MESHCulture Media-
dc.subject.MESHEpidermal Growth Factor-
dc.subject.MESHHumans-
dc.subject.MESHPlatelet-Derived Growth Factor-
dc.subject.MESHVascular Endothelial Growth Factor A-
dc.titleEffects of platelet lysate preparations on the proliferation of HaCaT cells.-
dc.typeArticle-
dc.identifier.pmid24422195-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885772/-
dc.contributor.affiliatedAuthor임, 영애-
dc.contributor.affiliatedAuthor이, 위교-
dc.contributor.affiliatedAuthor김, 철호-
dc.type.localJournal Papers-
dc.identifier.doi10.3343/alm.2014.34.1.43-
dc.citation.titleAnnals of laboratory medicine-
dc.citation.volume34-
dc.citation.number1-
dc.citation.date2014-
dc.citation.startPage43-
dc.citation.endPage50-
dc.identifier.bibliographicCitationAnnals of laboratory medicine, 34(1). : 43-50, 2014-
dc.identifier.eissn2234-3814-
dc.relation.journalidJ022343806-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
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