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Effects of platelet lysate preparations on the proliferation of HaCaT cells.
DC Field | Value | Language |
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dc.contributor.author | Baik, SY | - |
dc.contributor.author | Lim, YA | - |
dc.contributor.author | Kang, SJ | - |
dc.contributor.author | Ahn, SSSH | - |
dc.contributor.author | Lee, WG | - |
dc.contributor.author | Kim, CH | - |
dc.date.accessioned | 2016-11-18 | - |
dc.date.available | 2016-11-18 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2234-3806 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/12893 | - |
dc.description.abstract | BACKGROUND: Standard protocols are lacking for the preparation of platelet
lysates (PL) as an alternative to using fetal bovine serum as a cell culture supplement. This study aimed to establish optimum conditions for preparing PL for use in cell cultures. METHODS: Cell density in three pooled platelet concentrates (PC) were adjusted to 1x10(12)/L and 2x10(12)/L. PL was prepared from PC by 1 to 3 freeze-thaw (FT) cycles. HaCaT cells were cultured in media supplemented with 5% or 10% PL. Cell numbers were estimated using a Cell Counting Kit-8 (CCK-8; Dojindo Laboratories, Japan). Growth factors were quantified by using the Luminex 200 system (Luminex Corporation, USA). RESULTS: Cell proliferation rates in the presence of PLs were similar when prepared from PCs of both cell densities. The rates were higher in media containing 5% PL than 10% PL when prepared by two FT cycles. Concentrations of vascular endothelial growth factor (VEGF), platelet-derived growth factor-AB/BB (PDGF-AB/BB), PDGF-AA, and epidermal growth factor (EGF) were significantly higher in PL prepared from PC with a cell density of 2x10(12)/L than 1x10(12)/L PC. However, only VEGF and PDGF-AA concentrations in PLs were correlated with HaCaT cell counts. CONCLUSIONS: The 5% PL from PC with a cell density of 1x10(12)/L prepared by two FT cycles treatment was the most effective condition that supported steady HaCaT cell proliferation. Our finding may be useful for preparing PL-supplemented cell culture media. | - |
dc.language.iso | en | - |
dc.subject.MESH | Blood Platelets | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Culture Media | - |
dc.subject.MESH | Epidermal Growth Factor | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Platelet-Derived Growth Factor | - |
dc.subject.MESH | Vascular Endothelial Growth Factor A | - |
dc.title | Effects of platelet lysate preparations on the proliferation of HaCaT cells. | - |
dc.type | Article | - |
dc.identifier.pmid | 24422195 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3885772/ | - |
dc.contributor.affiliatedAuthor | 임, 영애 | - |
dc.contributor.affiliatedAuthor | 이, 위교 | - |
dc.contributor.affiliatedAuthor | 김, 철호 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.3343/alm.2014.34.1.43 | - |
dc.citation.title | Annals of laboratory medicine | - |
dc.citation.volume | 34 | - |
dc.citation.number | 1 | - |
dc.citation.date | 2014 | - |
dc.citation.startPage | 43 | - |
dc.citation.endPage | 50 | - |
dc.identifier.bibliographicCitation | Annals of laboratory medicine, 34(1). : 43-50, 2014 | - |
dc.identifier.eissn | 2234-3814 | - |
dc.relation.journalid | J022343806 | - |
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