Cited 0 times in Scipus Cited Count

Preparation of Extracellular Matrix Developed Using Porcine Articular Cartilage and In Vitro Feasibility Study of Porcine Articular Cartilage as an Anti-Adhesive Film

DC Field Value Language
dc.contributor.authorBaek, JH-
dc.contributor.authorKim, K-
dc.contributor.authorYang, SS-
dc.contributor.authorPark, SH-
dc.contributor.authorSong, BR-
dc.contributor.authorYun, HW-
dc.contributor.authorJeong, SI-
dc.contributor.authorKim, YJ-
dc.contributor.authorMin, BH-
dc.contributor.authorKim, MS-
dc.date.accessioned2018-06-12T04:30:30Z-
dc.date.available2018-06-12T04:30:30Z-
dc.date.issued2016-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/15299-
dc.description.abstractIn this study, we examined whether porcine articular cartilage (PAC) is a suitable and effective anti-adhesive material. PAC, which contained no non-collagenous tissue components, was collected by mechanical manipulation and decellularization of porcine knee cartilage. The PAC film for use as an anti-adhesive barrier was easily shaped into various sizes using homemade silicone molds. The PAC film was cross-linked to study the usefulness of the anti-adhesive barrier shape. The cross-linked PAC (Cx-PAC) film showed more stable physical properties over extended periods compared to uncross-linked PAC (UnCx-PAC) film. To control the mechanical properties, Cx-PAC film was thermally treated at 45 degrees C or 65 degrees C followed by incubation at room temperature. The Cx-PAC films exhibited varying enthalpies, ultimate tensile strength values, and contact angles before and after thermal treatment and after incubation at room temperature. Next, to examine the anti-adhesive properties, human umbilical vein endothelial cells (HUVECs) were cultured on Cx-PAC and thermal-treated Cx-PAC films. Scanning electron microscopy, fluorescence, and MTT assays showed that HUVECs were well adhered to the surface of the plate and proliferated, indicating no inhibition of the attachment and proliferation of HUVECs. In contrast, Cx-PAC and thermal-treated Cx-PAC exhibited little and/or no cell attachment and proliferation because of the inhibition effect on HUVECs. In conclusion, we successfully developed a Cx-PAC film with controllable mechanical properties that can be used as an anti-adhesive barrier.-
dc.language.isoen-
dc.subject.MESHAnti-adhesive film-
dc.subject.MESHCross-linking-
dc.subject.MESHIncubation-
dc.subject.MESHPorcine articular cartilage-
dc.subject.MESHThermal treatment-
dc.titlePreparation of Extracellular Matrix Developed Using Porcine Articular Cartilage and In Vitro Feasibility Study of Porcine Articular Cartilage as an Anti-Adhesive Film-
dc.typeArticle-
dc.identifier.pmid28787849-
dc.contributor.affiliatedAuthor김, 영직-
dc.contributor.affiliatedAuthor민, 병현-
dc.type.localJournal Papers-
dc.identifier.doi10.3390/ma9010049-
dc.citation.titleMaterials (Basel, Switzerland)-
dc.citation.volume9-
dc.citation.number1-
dc.citation.date2016-
dc.citation.startPage49-
dc.citation.endPage49-
dc.identifier.bibliographicCitationMaterials (Basel, Switzerland), 9(1). : 49-49, 2016-
dc.identifier.eissn1996-1944-
dc.relation.journalidJ019961944-
Appears in Collections:
Journal Papers > Research Organization > Cell Therapy Center
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
Files in This Item:
28787849.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse