Cited 0 times in Scipus Cited Count

Three-Dimensional Hepatocellular Carcinoma/Fibroblast Model on a Nanofibrous Membrane Mimics Tumor Cell Phenotypic Changes and Anticancer Drug Resistance

DC Field Value Language
dc.contributor.authorLe, BD-
dc.contributor.authorKang, D-
dc.contributor.authorYun, S-
dc.contributor.authorJeong, YH-
dc.contributor.authorKwak, JY-
dc.contributor.authorYoon, S-
dc.contributor.authorJin, S-
dc.date.accessioned2019-11-13T00:17:44Z-
dc.date.available2019-11-13T00:17:44Z-
dc.date.issued2018-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/16732-
dc.description.abstractThree-dimensional (3D) in vitro tissue or organ models can effectively mimic the complex microenvironment of many types of human tissues for medical applications. Unfortunately, development of 3D cancer models, which involve cancer/stromal cells in a 3D environment, has remained elusive due to the extreme complexity of the tumor microenvironment (TME) and the stepwise progression of human cancer. Here, we developed hepatocellular carcinoma (HCC) models, which consist of fibroblasts as stromal cells, HCC cells, and a nanofibrous membrane to mimic the complex TME. The 3D HCC models were fabricated using three distinct culture methods: cancer cells grown directly on the nanofibrous membrane (mono model), fibroblasts covering the nanofibrous membrane (layer model), and both cancer cells and fibroblasts grown on the nanofibrous membrane (mixed model). Interestingly, the mono model and layer model showed similar tissue structures, whereas the mixed model resulted in phenotypic changes to the cancer cells. Further analysis demonstrated that the mixed models promoted the expression of fibronectin and vimentin, and showed higher resistance to anticancer drugs compared with the other models. Thus, our 3D HCC model could be utilized for testing efficient anticancer therapies at various stages of cancer, with potential application to different tumor types.-
dc.language.isoen-
dc.titleThree-Dimensional Hepatocellular Carcinoma/Fibroblast Model on a Nanofibrous Membrane Mimics Tumor Cell Phenotypic Changes and Anticancer Drug Resistance-
dc.typeArticle-
dc.identifier.pmid29370123-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5852456/-
dc.subject.keywordhepatocellular carcinoma-
dc.subject.keywordnanofibrous membrane-
dc.subject.keywordtumor microenvironment-
dc.contributor.affiliatedAuthor곽, 종영-
dc.type.localJournal Papers-
dc.identifier.doi10.3390/nano8020064-
dc.citation.titleNanomaterials (Basel, Switzerland)-
dc.citation.volume8-
dc.citation.number2-
dc.citation.date2018-
dc.citation.startPageE64-
dc.citation.endPageE64-
dc.identifier.bibliographicCitationNanomaterials (Basel, Switzerland), 8(2). : E64-E64, 2018-
dc.identifier.eissn2079-4991-
dc.relation.journalidJ020794991-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Files in This Item:
29370123.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse