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Ionizing radiation induces astrocyte gliosis through microglia activation.

DC Field Value Language
dc.contributor.authorHwang, SY-
dc.contributor.authorJung, JS-
dc.contributor.authorKim, TH-
dc.contributor.authorLim, SJ-
dc.contributor.authorOh, ES-
dc.contributor.authorKim, JY-
dc.contributor.authorJi, KA-
dc.contributor.authorJoe, EH-
dc.contributor.authorCho, KH-
dc.contributor.authorHan, IO-
dc.date.accessioned2011-03-23T07:25:35Z-
dc.date.available2011-03-23T07:25:35Z-
dc.date.issued2006-
dc.identifier.issn0969-9961-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/1889-
dc.description.abstractThe aim of this study was to investigate the role of microglia in radiation-induced astrocyte gliosis. We found that a single dose of 15 Gy radiation to a whole rat brain increased immunostaining of glial fibrillary acidic protein in astrocytes 6 h later, and even more so 24 h later, indicating the initiation of gliosis. While irradiation of cultured rat astrocytes had little effect, irradiation of microglia-astrocyte mixed-cultures displayed altered astrocyte phenotype into more processed, which is another characteristic of gliosis. Experiments using microglia-conditioned media indicated this astrocyte change was due to factors released from irradiated microglia. Irradiation of cultured mouse microglial cells induced a dose-dependent increase in mRNA levels for cyclooxygenase-2 (COX-2), interleukin (IL)-1beta, IL-6, IL-18, tumor necrosis factor-alpha and interferon-gamma-inducible protein-10, which are usually associated with microglia activation. Consistent with these findings, irradiation of microglia activated NF-kappaB, a transcription factor that regulates microglial activation. Addition of prostaglandin E2 (PGE2: a metabolic product of the COX-2 enzyme) to primary cultured rat astrocytes resulted in phenotypic changes similar to those observed in mixed-culture experiments. Therefore, it appears that PGE(2) released from irradiated microglia is a key mediator of irradiation-induced gliosis or astrocyte phenotype change. These data suggest that radiation-induced microglial activation and resultant production of PGE2 seems to be associated with an underlying cause of inflammatory complications associated with radiation therapy for malignant gliomas.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAstrocytes-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHCulture Media, Conditioned-
dc.subject.MESHCyclooxygenase 2-
dc.subject.MESHCyclooxygenase Inhibitors-
dc.subject.MESHDinoprostone-
dc.subject.MESHElectrophoretic Mobility Shift Assay-
dc.subject.MESHGlial Fibrillary Acidic Protein-
dc.subject.MESHGliosis-
dc.subject.MESHImmunoblotting-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMice-
dc.subject.MESHMicroglia-
dc.subject.MESHNitrobenzenes-
dc.subject.MESHRNA, Messenger-
dc.subject.MESHRadiation, Ionizing-
dc.subject.MESHRats-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSulfonamides-
dc.titleIonizing radiation induces astrocyte gliosis through microglia activation.-
dc.typeArticle-
dc.identifier.pmid16202616-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0969-9961(05)00232-9-
dc.contributor.affiliatedAuthor조, 은혜-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.nbd.2005.08.006-
dc.citation.titleNeurobiology of disease-
dc.citation.volume21-
dc.citation.number3-
dc.citation.date2006-
dc.citation.startPage457-
dc.citation.endPage467-
dc.identifier.bibliographicCitationNeurobiology of disease, 21(3). : 457-467, 2006-
dc.identifier.eissn1095-953X-
dc.relation.journalidJ009699961-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
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