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Extracts from the Perilla frutescens var. (Odash.) Kudo leaves have anti-tumor effects on the breast cancer cell via suppression of the YAP activity
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 모, 정순 | - |
| dc.contributor.author | 김, 초롱 | - |
| dc.date.accessioned | 2021-01-06T02:34:35Z | - |
| dc.date.available | 2021-01-06T02:34:35Z | - |
| dc.date.issued | 2020 | - |
| dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/19220 | - |
| dc.description.abstract | 비공개 | - |
| dc.description.tableofcontents | I. INTRODUCTION 1
II. MATERIAL AND METHODS 4 A. Cell culture and transfection 4 B. Preparation of Perilla frutescens extracts(PLE) 4 C. Reagents and antibodies 4 D. High performance liquid chromatography(HPLC) 5 E. Western blot and immunoprecipitation 5 F. Luciferase assay 6 G. RNA isolation and real-time quantitative polymerase chain reaction (RT-qPCR) 6 H. Retrovirus infection and stable cell lines 7 I. Wound healing assay 7 J. Cell viability assay 8 K. Clonogenic growth assay 8 L. Generation of knock-out cells 9 M. Statistical analysis 9 III. RESULTS 10 A. Identification of rosmarinic acid in Perilla frutescens by HPLC-UV analysis 10 B. Effects of PLE on cytotoxicity leads to cell apoptosis in MDA-MB-231 and BT549 TNBC 13 C. PLE increases YAP phosphorylation and reduces YAP/TAZ-TEAD-mediated transcriptional activity 16 D. PLE induces YAP phosphorylation via LATS1/2-dependent mechanism, but not MST1/2 20 E. PLE blocks cell proliferation and migration of human breast cancer cells by repressing YAP activity 23 IV. DISCUSSION 26 V. REFERENCE 29 | - |
| dc.language.iso | en | - |
| dc.title | Extracts from the Perilla frutescens var. (Odash.) Kudo leaves have anti-tumor effects on the breast cancer cell via suppression of the YAP activity | - |
| dc.type | Thesis | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000030307 | - |
| dc.subject.keyword | Hippo pathway | - |
| dc.subject.keyword | LATS1/2 | - |
| dc.subject.keyword | YAP | - |
| dc.subject.keyword | Perilla frutescens | - |
| dc.subject.keyword | triple negative breast cancer | - |
| dc.description.degree | Master | - |
| dc.contributor.department | 대학원 의생명과학과 | - |
| dc.contributor.affiliatedAuthor | 김, 초롱 | - |
| dc.date.awarded | 2020 | - |
| dc.type.local | Theses | - |
| dc.citation.date | 2020 | - |
| dc.embargo.liftdate | 9999-12-31 | - |
| dc.embargo.terms | 9999-12-31 | - |
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- Theses > Graduate School of Biomedical Sciences > Master
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