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Biophysical determinants of toluene diisocyanate antigenicity associated with exposure and asthma.
DC Field | Value | Language |
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dc.contributor.author | Ye, YM | - |
dc.contributor.author | Kim, CW | - |
dc.contributor.author | Kim, HR | - |
dc.contributor.author | Kim, HM | - |
dc.contributor.author | Suh, CH | - |
dc.contributor.author | Nahm, DH | - |
dc.contributor.author | Park, HS | - |
dc.contributor.author | Redlich, CA | - |
dc.contributor.author | Wisnewski, AV | - |
dc.date.accessioned | 2011-03-31T05:04:42Z | - |
dc.date.available | 2011-03-31T05:04:42Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0091-6749 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/2085 | - |
dc.description.abstract | BACKGROUND: Toluene diisocyanate (TDI), a widely used aromatic diisocyanate with the potential to cause asthma, reacts with albumin in the airway fluid, which acts as a carrier protein for chemical presentation to the immune system. Structural elucidation of TDI-albumin conjugates is crucial to understanding the human immune response to TDI exposure.
OBJECTIVE: Investigate the dependence of TDI's antigenicity on the biophysics of exposure and its association with TDI asthma. METHODS: Toluene diisocyanate-albumin conjugates were generated by exposing albumin to TDI in liquid or vapor phase (liquid or vapor TDI-albumin, respectively). Conjugates were characterized by native gel electrophoresis and matrix-assisted laser desorption/ionization-mass spectrometry, and used as antigens in ELISA assays for serum specific-IgE and IgG. RESULTS: The physical phase of TDI (vapor vs liquid) affects the formation of TDI-albumin conjugates, with measurable differences in the amount of TDI per albumin molecule, migration in native gels, matrix-assisted laser desorption/ionization-mass spectrometry mass/charge spectra, and antigenicity. Vapor TDI-albumin conjugates were recognized by IgE from 44% of subjects with TDI asthma, whereas liquid TDI-albumin conjugates are recognized by IgE from only 17% of these patients. A significant (P < .05) association between TDI exposure and vapor TDI-albumin specific serum IgG was also observed. CONCLUSION: Biophysics of TDI exposure substantially affects formation of TDI-albumin conjugates recognized by the immune system in association with exposure and asthma. CLINICAL IMPLICATIONS: The data suggest that serology may help identify TDI asthmatics and exposed workers if the appropriate form of TDI is used as the antigenic basis for analysis. | - |
dc.language.iso | en | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Albumins | - |
dc.subject.MESH | Antigens | - |
dc.subject.MESH | Asthma | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin E | - |
dc.subject.MESH | Immunoglobulin G | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Toluene 2,4-Diisocyanate | - |
dc.title | Biophysical determinants of toluene diisocyanate antigenicity associated with exposure and asthma. | - |
dc.type | Article | - |
dc.identifier.pmid | 17030242 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0091-6749(06)01379-0 | - |
dc.contributor.affiliatedAuthor | 예, 영민 | - |
dc.contributor.affiliatedAuthor | 서, 창희 | - |
dc.contributor.affiliatedAuthor | 남, 동호 | - |
dc.contributor.affiliatedAuthor | 박, 해심 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.jaci.2006.06.026 | - |
dc.citation.title | The Journal of allergy and clinical immunology | - |
dc.citation.volume | 118 | - |
dc.citation.number | 4 | - |
dc.citation.date | 2006 | - |
dc.citation.startPage | 885 | - |
dc.citation.endPage | 891 | - |
dc.identifier.bibliographicCitation | The Journal of allergy and clinical immunology, 118(4). : 885-891, 2006 | - |
dc.identifier.eissn | 1097-6825 | - |
dc.relation.journalid | J000916749 | - |
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