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Sphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening
DC Field | Value | Language |
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dc.contributor.author | Shin, K | - |
dc.contributor.author | Shin, H | - |
dc.contributor.author | Cho, HJ | - |
dc.contributor.author | Kang, H | - |
dc.contributor.author | Lee, JK | - |
dc.contributor.author | Seo, YJ | - |
dc.contributor.author | Shin, YJ | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Koo, H | - |
dc.contributor.author | Kong, DS | - |
dc.contributor.author | Seol, HJ | - |
dc.contributor.author | Lee, JI | - |
dc.contributor.author | Lee, HW | - |
dc.contributor.author | Nam, DH | - |
dc.date.accessioned | 2022-11-29T01:43:16Z | - |
dc.date.available | 2022-11-29T01:43:16Z | - |
dc.date.issued | 2020 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/22948 | - |
dc.description.abstract | Diffusely infiltrating gliomas (DIGs) are difficult to completely resect and are associated with a high rate of tumor relapse and progression from low- to high-grade glioma. In particular, optimized short-term culture-enriching patient-derived glioma stem cells (GSCs) are essential for customizing the therapeutic strategy based on clinically feasible in vitro drug screening for a wide range of DIGs, owing to the high inter-tumoral heterogeneity. Herein, we constructed a novel high-throughput culture condition screening platform called 'GFSCAN', which evaluated the cellular growth rates of GSCs for each DIG sample in 132 serum-free combinations, using 13 previously reported growth factors closely associated with glioma aggressiveness. In total, 72 patient-derived GSCs with available genomic profiles were tested in GFSCAN to explore the association between cellular growth rates in specific growth factor combinations and genomic/molecular backgrounds, including isocitrate dehydrogenase 1 (IDH1) mutation, chromosome arm 1p and 19q co-deletion, ATRX chromatin remodeler alteration, and transcriptional subtype. GSCs were clustered according to the dependency on epidermal growth factor and basic fibroblast growth factor (E&F), and isocitrate dehydrogenase 1 (IDH1) wild-type GSCs showed higher E&F dependencies than IDH1 mutant GSCs. More importantly, we elucidated optimal combinations for IDH1 mutant glioblastoma and lower grade glioma GSCs with low dependencies on E&F, which could be an aid in clinical decision-making for these DIGs. Thus, we demonstrated the utility of GFSCAN in personalizing in vitro cultivation to nominate personalized therapeutic options, in a clinically relevant time frame, for individual DIG patients, where standard clinical options have been exhausted. | - |
dc.language.iso | en | - |
dc.title | Sphere-Forming Culture for Expanding Genetically Distinct Patient-Derived Glioma Stem Cells by Cellular Growth Rate Screening | - |
dc.type | Article | - |
dc.identifier.pmid | 32120790 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7139415 | - |
dc.subject.keyword | diffuse infiltrating glioma | - |
dc.subject.keyword | personalized medicine | - |
dc.subject.keyword | glioma stem cells | - |
dc.subject.keyword | short-term cultivation screening platform | - |
dc.subject.keyword | growth factor | - |
dc.subject.keyword | genomic profiling | - |
dc.contributor.affiliatedAuthor | Lee, JK | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.3390/cancers12030549 | - |
dc.citation.title | Cancers | - |
dc.citation.volume | 12 | - |
dc.citation.number | 3 | - |
dc.citation.date | 2020 | - |
dc.citation.startPage | 549 | - |
dc.citation.endPage | 549 | - |
dc.identifier.bibliographicCitation | Cancers, 12(3). : 549-549, 2020 | - |
dc.identifier.eissn | 2072-6694 | - |
dc.relation.journalid | J020726694 | - |
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