Cited 0 times in Scipus Cited Count

Effect of silica-coated magnetic nanoparticles on rigidity sensing of human embryonic kidney cells

Authors
Ketebo, AA | Shin, TH  | Jun, M | Lee, G  | Park, S
Citation
Journal of nanobiotechnology, 18(1). : 170-170, 2020
Journal Title
Journal of nanobiotechnology
ISSN
1477-3155
Abstract
BACKGROUND: Nanoparticles (NPs) can enter cells and cause cellular dysfunction. For example, reactive oxygen species generated by NPs can damage the cytoskeleton and impair cellular adhesion properties. Previously, we reported that cell spreading and protrusion structures such as lamellipodia and filopodia was reduced when cells are treated with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate (MNPs@SiO2(RITC)), even at 0.1 mug/muL. These protruded structures are involved in a cell's rigidity sensing, but how these NPs affect rigidity sensing is unknown. RESULTS: Here, we report that the rigidity sensing of human embryonic kidney (HEK293) cells was impaired even at 0.1 mug/muL of MNPs@SiO2(RITC). At this concentration, cells were unable to discern the stiffness difference between soft (5 kPa) and rigid (2 MPa) flat surfaces. The impairment of rigidity sensing was further supported by observing the disappearance of locally contracted elastomeric submicron pillars (900 nm in diameter, 2 mum in height, 24.21 nN/mum in stiffness k) under MNPs@SiO2(RITC) treated cells. A decrease in the phosphorylation of paxillin, which is involved in focal adhesion dynamics, may cause cells to be insensitive to stiffness differences when they are treated with MNPs@SiO2(RITC). CONCLUSIONS: Our results suggest that NPs may impair the rigidity sensing of cells even at low concentrations, thereby affecting cell adhesion and spreading.
Keywords

MeSH

DOI
10.1186/s12951-020-00730-2
PMID
33208165
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Ajou Authors
신, 태환  |  이, 광
Full Text Link
Files in This Item:
33208165.pdfDownload
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse