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Involvement of calcium-mediated apoptotic signals in H2O2-induced MIN6N8a cell death.
DC Field | Value | Language |
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dc.contributor.author | Choi, SE | - |
dc.contributor.author | Min, SH | - |
dc.contributor.author | Shin, HC | - |
dc.contributor.author | Kim, HE | - |
dc.contributor.author | Jung, MW | - |
dc.contributor.author | Kang, Y | - |
dc.date.accessioned | 2011-04-20T05:26:58Z | - |
dc.date.available | 2011-04-20T05:26:58Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0014-2999 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/2398 | - |
dc.description.abstract | Reactive oxygen species are believed to be the central mediators of beta-cell destruction that leads to type 1 and 2 diabetes, and calcium has been reported to be an important mediator of beta cell death. In the present study, the authors investigated whether Ca(2+) plays a role in hydrogen peroxide (H(2)O(2))-induced MIN6N8a mouse beta cell death. Treatment with low concentration H(2)O(2) (50 microM) was found to be sufficient to reduce MIN6N8a cell viability by 55%, largely via apoptosis. However, this H(2)O(2)-induced cell death was near completely blocked by pretreatment with BAPTA/AM (5 microM), a chelator of intracellular Ca(2+). Moreover, the intracellular calcium store channel blockers, such as, xestospongin c and ryanodine, significant protected cells from 50 microM H(2)O(2)-induced cell death and under extracellular Ca(2+)-free conditions, 50 microM H(2)O(2) elicited transient [Ca(2+)](i) increases. In addition, pharmacologic inhibitors of calpain, calcineurin, and calcium/calmodulin-dependent protein kinase II were found to have a protective effect on H(2)O(2)-induced death. Moreover, H(2)O(2)-induced apoptotic signals, such as c-JUN N-terminal kinase activation, cytochrome c release, caspase 3 activation, and poly (ADP-ribose) polymerase cleavage were all down-regulated by the intracellular Ca(2+) chelation. These findings show that [Ca(2+)](i) elevation, possibly due to release from intracellular calcium stores and the subsequent activation of Ca(2+)-mediated apoptotic signals, critically mediates low concentration H(2)O(2)-induced MIN6N8a cell death. These findings suggest that a breakdown of calcium homeostasis by low level of reactive oxygen species may be involved in beta cell destruction during diabetes development. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Calcineurin | - |
dc.subject.MESH | Calcium | - |
dc.subject.MESH | Calpain | - |
dc.subject.MESH | Caspase 3 | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Chelating Agents | - |
dc.subject.MESH | Egtazic Acid | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Enzyme Inhibitors | - |
dc.subject.MESH | Hydrogen Peroxide | - |
dc.subject.MESH | Insulinoma | - |
dc.subject.MESH | Intracellular Fluid | - |
dc.subject.MESH | JNK Mitogen-Activated Protein Kinases | - |
dc.subject.MESH | Macrocyclic Compounds | - |
dc.subject.MESH | Oxazoles | - |
dc.subject.MESH | Oxidants | - |
dc.subject.MESH | Poly(ADP-ribose) Polymerases | - |
dc.subject.MESH | Ryanodine | - |
dc.subject.MESH | Signal Transduction | - |
dc.title | Involvement of calcium-mediated apoptotic signals in H2O2-induced MIN6N8a cell death. | - |
dc.type | Article | - |
dc.identifier.pmid | 16934799 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0014-2999(06)00639-X | - |
dc.contributor.affiliatedAuthor | 최, 성이 | - |
dc.contributor.affiliatedAuthor | 정, 민환 | - |
dc.contributor.affiliatedAuthor | 강, 엽 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.ejphar.2006.06.016 | - |
dc.citation.title | European journal of pharmacology | - |
dc.citation.volume | 547 | - |
dc.citation.number | 1-3 | - |
dc.citation.date | 2006 | - |
dc.citation.startPage | 1 | - |
dc.citation.endPage | 9 | - |
dc.identifier.bibliographicCitation | European journal of pharmacology, 547(1-3). : 1-9, 2006 | - |
dc.identifier.eissn | 1879-0712 | - |
dc.relation.journalid | J000142999 | - |
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