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Immunoglobulin can be functionally regulated by protein carboxylmethylation in Fc region.

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dc.contributor.authorPark, JS-
dc.contributor.authorCho, JY-
dc.contributor.authorKim, SS-
dc.contributor.authorBae, HJ-
dc.contributor.authorHan, JW-
dc.contributor.authorLee, HW-
dc.contributor.authorHong, SY-
dc.date.accessioned2011-04-22T02:35:09Z-
dc.date.available2011-04-22T02:35:09Z-
dc.date.issued2006-
dc.identifier.issn0253-6269-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2424-
dc.description.abstractProtein carboxylmethylation methylates the free carboxyl groups in various substrate proteins by protein carboxyl O-methyltransferase (PCMT) and is one of the post-translational modifications. There have been many studies on protein carboxylmethylation. However, the precise functional role in mammalian systems is unclear. In this study, immunoglobulin, a specific form of gamma-globulin, which is a well-known substrate for PCMT, was chosen to investigate the regulatory roles of protein carboxylmethylation in the immune system. It was found that the anti-BSA antibody could be carboxylmethylated via spleen PCMT to a level similar to gamma-globulin. This carboxylmethylation increased the hydrophobicity of the anti-BSA antibody up to 11.4%, and enhanced the antigen-binding activity of this antibody up to 24.6%. In particular, the Fc region showed a higher methyl accepting capacity with 80% of the whole structure level. According to the amino acid sequence alignment, indeed, 7 aspartic acids and 5 glutamic acids, as potential carboxylmethylation sites, were found to be conserved in the Fc portion in the human, mouse and rabbit. The carboxylmethylation of the anti-BSA antibody was reversibly demethylated under a higher pH and long incubation time. Therefore, these results suggest that protein carboxylmethylation may reversibly regulate the antibody-mediated immunological events via the Fc region.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies-
dc.subject.MESHBinding Sites, Antibody-
dc.subject.MESHImmunoglobulin Fab Fragments-
dc.subject.MESHImmunoglobulin Fc Fragments-
dc.subject.MESHImmunoglobulin G-
dc.subject.MESHMethylation-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHPapain-
dc.subject.MESHProtein O-Methyltransferase-
dc.subject.MESHRabbits-
dc.subject.MESHSequence Alignment-
dc.subject.MESHSerum Albumin, Bovine-
dc.titleImmunoglobulin can be functionally regulated by protein carboxylmethylation in Fc region.-
dc.typeArticle-
dc.identifier.pmid16756083-
dc.contributor.affiliatedAuthor김, 성수-
dc.type.localJournal Papers-
dc.citation.titleArchives of pharmacal research-
dc.citation.volume29-
dc.citation.number5-
dc.citation.date2006-
dc.citation.startPage384-
dc.citation.endPage393-
dc.identifier.bibliographicCitationArchives of pharmacal research, 29(5). : 384-393, 2006-
dc.relation.journalidJ002536269-
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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