Cited 0 times in Scipus Cited Count

ATF3 plays a key role in Kdo2-lipid A-induced TLR4-dependent gene expression via NF-κB activation.

DC Field Value Language
dc.contributor.authorKim, EY-
dc.contributor.authorShin, HY-
dc.contributor.authorKim, JY-
dc.contributor.authorKim, DG-
dc.contributor.authorChoi, YM-
dc.contributor.authorKwon, HK-
dc.contributor.authorRhee, DK-
dc.contributor.authorKim, YS-
dc.contributor.authorChoi, S-
dc.date.accessioned2011-05-31T01:06:17Z-
dc.date.available2011-05-31T01:06:17Z-
dc.date.issued2010-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/2720-
dc.description.abstractBACKGROUND: Activating transcription factor 3 (ATF3) is a negative regulator of proinflammatory cytokine expression in macrophages, and ATF3 deficient mice are more susceptible to endotoxic shock. This study addresses the role of ATF3 in the Kdo(2)-Lipid A-induced Toll-like receptor 4 (TLR4) signaling pathway in mouse embryonic fibroblasts (MEF). Kdo(2)-Lipid A upregulates ATF3 expression in wild type MEF cells and induces both nuclear factor kappa B (NF-κB) and c-Jun N-terminal kinase (JNK) activation via the TLR4 signaling pathway, while neither of these pathways is activated in ATF3-/- MEF cells. Interestingly, in contrast to Kdo(2)-Lipid A, the activation of both NF-κB and JNK by TNF-α was normal in ATF3-/- MEF cells.



METHODOLOGY/PRINCIPAL FINDINGS: We found that several genes were dramatically upregulated in ATF3+/+ MEF cells in response to Kdo(2)-Lipid A treatment, while little difference was observed in the ATF3-/- MEF cells. However, we also found that the signal intensities of IκBζ in ATF3-/- MEF cells were substantially higher than those in wild type MEF cells upon microarray analyses, and upregulated IκBζ expression was detected in the cytosol fraction.



CONCLUSIONS/SIGNIFICANCE: Our findings indicate that ATF3 deficiency affects Kdo(2)-Lipid A-induced TLR4 signaling pathways in MEF cells, that it may upregulate IκBζ expression and that the high levels of IκBζ expression in ATF3-/- cells disrupts Kdo(2)-Lipid A-mediated signaling pathways.
-
dc.language.isoen-
dc.titleATF3 plays a key role in Kdo2-lipid A-induced TLR4-dependent gene expression via NF-κB activation.-
dc.typeArticle-
dc.identifier.pmid21152039-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996292/-
dc.contributor.affiliatedAuthor김, 유선-
dc.type.localJournal Papers-
dc.identifier.doi10.1371/journal.pone.0014181-
dc.citation.titlePloS one-
dc.citation.volume5-
dc.citation.number12-
dc.citation.date2010-
dc.citation.startPagee14181-
dc.citation.endPagee14181-
dc.identifier.bibliographicCitationPloS one, 5(12). : e14181-e14181, 2010-
dc.identifier.eissn1932-6203-
dc.relation.journalidJ019326203-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
Files in This Item:
21152039.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse