Cited 0 times in
ATF3 plays a key role in Kdo2-lipid A-induced TLR4-dependent gene expression via NF-κB activation.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, EY | - |
dc.contributor.author | Shin, HY | - |
dc.contributor.author | Kim, JY | - |
dc.contributor.author | Kim, DG | - |
dc.contributor.author | Choi, YM | - |
dc.contributor.author | Kwon, HK | - |
dc.contributor.author | Rhee, DK | - |
dc.contributor.author | Kim, YS | - |
dc.contributor.author | Choi, S | - |
dc.date.accessioned | 2011-05-31T01:06:17Z | - |
dc.date.available | 2011-05-31T01:06:17Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/2720 | - |
dc.description.abstract | BACKGROUND: Activating transcription factor 3 (ATF3) is a negative regulator of proinflammatory cytokine expression in macrophages, and ATF3 deficient mice are more susceptible to endotoxic shock. This study addresses the role of ATF3 in the Kdo(2)-Lipid A-induced Toll-like receptor 4 (TLR4) signaling pathway in mouse embryonic fibroblasts (MEF). Kdo(2)-Lipid A upregulates ATF3 expression in wild type MEF cells and induces both nuclear factor kappa B (NF-κB) and c-Jun N-terminal kinase (JNK) activation via the TLR4 signaling pathway, while neither of these pathways is activated in ATF3-/- MEF cells. Interestingly, in contrast to Kdo(2)-Lipid A, the activation of both NF-κB and JNK by TNF-α was normal in ATF3-/- MEF cells.
METHODOLOGY/PRINCIPAL FINDINGS: We found that several genes were dramatically upregulated in ATF3+/+ MEF cells in response to Kdo(2)-Lipid A treatment, while little difference was observed in the ATF3-/- MEF cells. However, we also found that the signal intensities of IκBζ in ATF3-/- MEF cells were substantially higher than those in wild type MEF cells upon microarray analyses, and upregulated IκBζ expression was detected in the cytosol fraction. CONCLUSIONS/SIGNIFICANCE: Our findings indicate that ATF3 deficiency affects Kdo(2)-Lipid A-induced TLR4 signaling pathways in MEF cells, that it may upregulate IκBζ expression and that the high levels of IκBζ expression in ATF3-/- cells disrupts Kdo(2)-Lipid A-mediated signaling pathways. | - |
dc.language.iso | en | - |
dc.title | ATF3 plays a key role in Kdo2-lipid A-induced TLR4-dependent gene expression via NF-κB activation. | - |
dc.type | Article | - |
dc.identifier.pmid | 21152039 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996292/ | - |
dc.contributor.affiliatedAuthor | 김, 유선 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1371/journal.pone.0014181 | - |
dc.citation.title | PloS one | - |
dc.citation.volume | 5 | - |
dc.citation.number | 12 | - |
dc.citation.date | 2010 | - |
dc.citation.startPage | e14181 | - |
dc.citation.endPage | e14181 | - |
dc.identifier.bibliographicCitation | PloS one, 5(12). : e14181-e14181, 2010 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.relation.journalid | J019326203 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.