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Nitric oxide negatively regulates c-Jun N-terminal kinase/stress-activated protein kinase by means of S-nitrosylation.
DC Field | Value | Language |
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dc.contributor.author | Park, HS | - |
dc.contributor.author | Huh, SH | - |
dc.contributor.author | Kim, MS | - |
dc.contributor.author | Lee, SH | - |
dc.contributor.author | Choi, EJ | - |
dc.date.accessioned | 2011-07-27 | - |
dc.date.available | 2011-07-27 | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/3611 | - |
dc.description.abstract | NO, produced from l-arginine in a reaction catalyzed by NO synthase, is an endogenous free radical with multiple functions in mammalian cells. Here, we demonstrate that endogenously produced NO can suppress c-Jun N-terminal kinase (JNK) activation in intact cells. Treatment of BV-2 murine microglial cells with IFN-gamma induced endogenous NO production, concomitantly suppressing JNK1 activation. Similarly, IFN-gamma induced suppression of JNK1 activation in RAW264.7 murine macrophage cells and rat alveolar macrophages. The IFN-gamma-induced suppression of JNK1 activation in BV-2, RAW264.7, or rat alveolar macrophage cells was completely prevented by N(G)-nitro-l-arginine, a NO synthase inhibitor. Interestingly, the IFN-gamma-induced suppression of JNK1 activation was not affected by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, an inhibitor of guanylyl cyclase. 8-Bromo-cGMP, a membrane-permeant analogue of cGMP, did not change JNK1 activation in intact cells either. In contrast, S-nitro-N-acetyl-dl-penicillamine (SNAP), a NO donor, inhibited JNK1 activity in vitro. Furthermore, a thiol reducing agent, DTT, reversed not only the in vitro inhibition of JNK1 activity by SNAP but also the in vivo suppression of JNK1 activity by IFN-gamma. Substitution of serine for cysteine-116 in JNK1 abolished the inhibitory effect of IFN-gamma or SNAP on JNK1 activity in vivo or in vitro, respectively. Moreover, IFN-gamma enhanced endogenous S-nitrosylation of JNK1 in RAW264.7 cells. Collectively, our data suggest that endogenous NO mediates the IFN-gamma-induced suppression of JNK1 activation in macrophage cells by means of a thiol-redox mechanism. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cyclic GMP | - |
dc.subject.MESH | Cysteine | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interferon-gamma | - |
dc.subject.MESH | JNK Mitogen-Activated Protein Kinases | - |
dc.subject.MESH | MAP Kinase Kinase 4 | - |
dc.subject.MESH | MAP Kinase Kinase Kinase 1 | - |
dc.subject.MESH | Macrophages | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 8 | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase Kinases | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases | - |
dc.subject.MESH | Nitric Oxide | - |
dc.subject.MESH | Nitric Oxide Donors | - |
dc.subject.MESH | Oxidation-Reduction | - |
dc.subject.MESH | Penicillamine | - |
dc.subject.MESH | Protein-Serine-Threonine Kinases | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Sulfhydryl Compounds | - |
dc.title | Nitric oxide negatively regulates c-Jun N-terminal kinase/stress-activated protein kinase by means of S-nitrosylation. | - |
dc.type | Article | - |
dc.identifier.pmid | 11121042 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC18927/ | - |
dc.contributor.affiliatedAuthor | 이, 수환 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1073/pnas.97.26.14382 | - |
dc.citation.title | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.citation.volume | 97 | - |
dc.citation.number | 26 | - |
dc.citation.date | 2000 | - |
dc.citation.startPage | 14382 | - |
dc.citation.endPage | 14387 | - |
dc.identifier.bibliographicCitation | Proceedings of the National Academy of Sciences of the United States of America, 97(26). : 14382-14387, 2000 | - |
dc.identifier.eissn | 1091-6490 | - |
dc.relation.journalid | J000278424 | - |
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