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Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway.
DC Field | Value | Language |
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dc.contributor.author | Yun, J | - |
dc.contributor.author | Chae, HD | - |
dc.contributor.author | Choi, TS | - |
dc.contributor.author | Kim, EH | - |
dc.contributor.author | Bang, YJ | - |
dc.contributor.author | Chung, J | - |
dc.contributor.author | Choi, KS | - |
dc.contributor.author | Mantovani, R | - |
dc.contributor.author | Shin, DY | - |
dc.date.accessioned | 2011-07-28T04:51:30Z | - |
dc.date.available | 2011-07-28T04:51:30Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/3661 | - |
dc.description.abstract | Recent studies have suggested that the NF-Y transcription factor is involved in transcription repression of the cell cycle regulatory genes in a response to p53 induction or DNA damage. Here we demonstrate the cdk2-dependent phosphorylation of NF-Y and its involvement in transcription repression by the p53-p21 signaling pathway. Cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y. Cyclin A-cdk2 appears to associate with NF-Y both in vitro and in vivo. Furthermore, YA protein is phosphorylated in parallel with a cell cycle-dependent activation of cdk2 kinase and cyclin A expression. YA phosphorylation is unnecessary for heterotrimer formation with the YB-YC dimer. However, NF-Y containing a phosphorylation-deficient mutant form of YA, YA-aa, has its DNA binding activity impaired. Consistently, YA-aa inhibits transcription activation of a NF-Y target promoter, cdc2, by cdk2. These results facilitate the elucidation of the regulatory mechanisms of cell cycle progression involving the p21-cdk2-NF-Y signaling pathway. | - |
dc.language.iso | en | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | CCAAT-Binding Factor | - |
dc.subject.MESH | CDC2-CDC28 Kinases | - |
dc.subject.MESH | Cell Cycle | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Nucleus | - |
dc.subject.MESH | Cyclin A | - |
dc.subject.MESH | Cyclin-Dependent Kinase 2 | - |
dc.subject.MESH | Cyclin-Dependent Kinase Inhibitor p21 | - |
dc.subject.MESH | Cyclins | - |
dc.subject.MESH | DNA Damage | - |
dc.subject.MESH | Dimerization | - |
dc.subject.MESH | Glutathione Transferase | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Mutation | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Plasmids | - |
dc.subject.MESH | Precipitin Tests | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Purines | - |
dc.subject.MESH | Serine | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Transfection | - |
dc.subject.MESH | Tumor Suppressor Protein p53 | - |
dc.title | Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway. | - |
dc.type | Article | - |
dc.identifier.pmid | 12857729 | - |
dc.identifier.url | http://www.jbc.org/cgi/pmidlookup?view=long&pmid=12857729 | - |
dc.contributor.affiliatedAuthor | 최, 경숙 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1074/jbc.M305178200 | - |
dc.citation.title | The Journal of biological chemistry | - |
dc.citation.volume | 278 | - |
dc.citation.number | 38 | - |
dc.citation.date | 2003 | - |
dc.citation.startPage | 36966 | - |
dc.citation.endPage | 36972 | - |
dc.identifier.bibliographicCitation | The Journal of biological chemistry, 278(38). : 36966-36972, 2003 | - |
dc.identifier.eissn | 1083-351X | - |
dc.relation.journalid | J000219258 | - |
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