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Cdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway.

DC Field Value Language
dc.contributor.authorYun, J-
dc.contributor.authorChae, HD-
dc.contributor.authorChoi, TS-
dc.contributor.authorKim, EH-
dc.contributor.authorBang, YJ-
dc.contributor.authorChung, J-
dc.contributor.authorChoi, KS-
dc.contributor.authorMantovani, R-
dc.contributor.authorShin, DY-
dc.date.accessioned2011-07-28T04:51:30Z-
dc.date.available2011-07-28T04:51:30Z-
dc.date.issued2003-
dc.identifier.issn0021-9258-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3661-
dc.description.abstractRecent studies have suggested that the NF-Y transcription factor is involved in transcription repression of the cell cycle regulatory genes in a response to p53 induction or DNA damage. Here we demonstrate the cdk2-dependent phosphorylation of NF-Y and its involvement in transcription repression by the p53-p21 signaling pathway. Cdk2 phosphorylates two serine residues near the DNA-binding domain of the YA subunit of NF-Y. Cyclin A-cdk2 appears to associate with NF-Y both in vitro and in vivo. Furthermore, YA protein is phosphorylated in parallel with a cell cycle-dependent activation of cdk2 kinase and cyclin A expression. YA phosphorylation is unnecessary for heterotrimer formation with the YB-YC dimer. However, NF-Y containing a phosphorylation-deficient mutant form of YA, YA-aa, has its DNA binding activity impaired. Consistently, YA-aa inhibits transcription activation of a NF-Y target promoter, cdc2, by cdk2. These results facilitate the elucidation of the regulatory mechanisms of cell cycle progression involving the p21-cdk2-NF-Y signaling pathway.-
dc.language.isoen-
dc.subject.MESHAmino Acid Sequence-
dc.subject.MESHCCAAT-Binding Factor-
dc.subject.MESHCDC2-CDC28 Kinases-
dc.subject.MESHCell Cycle-
dc.subject.MESHCell Line-
dc.subject.MESHCell Nucleus-
dc.subject.MESHCyclin A-
dc.subject.MESHCyclin-Dependent Kinase 2-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p21-
dc.subject.MESHCyclins-
dc.subject.MESHDNA Damage-
dc.subject.MESHDimerization-
dc.subject.MESHGlutathione Transferase-
dc.subject.MESHHumans-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHMutation-
dc.subject.MESHPhosphorylation-
dc.subject.MESHPlasmids-
dc.subject.MESHPrecipitin Tests-
dc.subject.MESHPromoter Regions, Genetic-
dc.subject.MESHProtein Binding-
dc.subject.MESHPurines-
dc.subject.MESHSerine-
dc.subject.MESHSignal Transduction-
dc.subject.MESHTime Factors-
dc.subject.MESHTransfection-
dc.subject.MESHTumor Suppressor Protein p53-
dc.titleCdk2-dependent phosphorylation of the NF-Y transcription factor and its involvement in the p53-p21 signaling pathway.-
dc.typeArticle-
dc.identifier.pmid12857729-
dc.identifier.urlhttp://www.jbc.org/cgi/pmidlookup?view=long&pmid=12857729-
dc.contributor.affiliatedAuthor최, 경숙-
dc.type.localJournal Papers-
dc.identifier.doi10.1074/jbc.M305178200-
dc.citation.titleThe Journal of biological chemistry-
dc.citation.volume278-
dc.citation.number38-
dc.citation.date2003-
dc.citation.startPage36966-
dc.citation.endPage36972-
dc.identifier.bibliographicCitationThe Journal of biological chemistry, 278(38). : 36966-36972, 2003-
dc.identifier.eissn1083-351X-
dc.relation.journalidJ000219258-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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