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Translocational inefficiency of intracellular proteins in senescence of human diploid fibroblasts.

DC Field Value Language
dc.contributor.authorLim, IK-
dc.contributor.authorHong, KW-
dc.contributor.authorKwak, IH-
dc.contributor.authorYoon, G-
dc.contributor.authorPark, SC-
dc.date.accessioned2011-08-08T02:13:39Z-
dc.date.available2011-08-08T02:13:39Z-
dc.date.issued2001-
dc.identifier.issn0077-8923-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/3699-
dc.description.abstractIn order to investigate signal transduction pathways and related changes of actin cytoskeleton organization in cellular senescence, H-ras double mutants--V12S35, V12G37, and V12C40--were constitutively expressed in human foreskin fibroblast (HDF). Senescent HDF cells as well as the H-ras mutant expressers accumulated p-Erk1/2 in the cytoplasm with increased MEK activity and failed to translocate it to nuclei on EGF stimulation. Senescent HDF cells, V12S35 and V12G37 expressers, revealed a failure to export actin fiber from nucleus to cytoplasm and also to form stress fibers. Perinuclear expression of Rac1 was prominent in the HDF cells and V12C40 expresser; however, in the V12S35 expresser, translocation of Rac1 from perinucleus to nucleus and strong expression of RhoA were obvious. In summary, the H-ras double mutant expressers induced premature senescence through the MEK pathway, accompanied by nuclear accumulation of actin and Rac1 proteins, cytoplasmic retention of p-Erk1/2, and marked induction of RhoA expression, suggesting the translocational inefficiency of the intracellular proteins in the senescent HDF cells.-
dc.language.isoen-
dc.subject.MESH3T3 Cells-
dc.subject.MESHActins-
dc.subject.MESHActive Transport, Cell Nucleus-
dc.subject.MESHAnimals-
dc.subject.MESHBlood Proteins-
dc.subject.MESHCell Aging-
dc.subject.MESHCell Nucleus-
dc.subject.MESHCell Surface Extensions-
dc.subject.MESHCyclin-Dependent Kinase Inhibitor p16-
dc.subject.MESHCytoplasm-
dc.subject.MESHCytoskeletal Proteins-
dc.subject.MESHCytoskeleton-
dc.subject.MESHDiploidy-
dc.subject.MESHFibroblasts-
dc.subject.MESHGenes, p16-
dc.subject.MESHGenes, p53-
dc.subject.MESHGenes, ras-
dc.subject.MESHHumans-
dc.subject.MESHMAP Kinase Kinase Kinase 1-
dc.subject.MESHMAP Kinase Signaling System-
dc.subject.MESHMale-
dc.subject.MESHMembrane Proteins-
dc.subject.MESHMice-
dc.subject.MESHMicrofilament Proteins-
dc.subject.MESHMitogen-Activated Protein Kinase 1-
dc.subject.MESHMitogen-Activated Protein Kinase 3-
dc.subject.MESHMitogen-Activated Protein Kinases-
dc.subject.MESHPhosphoproteins-
dc.subject.MESHProtein Isoforms-
dc.subject.MESHProtein-Serine-Threonine Kinases-
dc.subject.MESHProto-Oncogene Proteins p21(ras)-
dc.subject.MESHStress Fibers-
dc.subject.MESHTumor Suppressor Protein p53-
dc.subject.MESHrac1 GTP-Binding Protein-
dc.subject.MESHrhoA GTP-Binding Protein-
dc.titleTranslocational inefficiency of intracellular proteins in senescence of human diploid fibroblasts.-
dc.typeArticle-
dc.identifier.pmid11795508-
dc.identifier.urlhttp://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0077-8923&date=2001&volume=928&spage=176-
dc.contributor.affiliatedAuthor임, 인경-
dc.contributor.affiliatedAuthor윤, 계순-
dc.type.localJournal Papers-
dc.citation.titleAnnals of the New York Academy of Sciences-
dc.citation.volume928-
dc.citation.date2001-
dc.citation.startPage176-
dc.citation.endPage181-
dc.identifier.bibliographicCitationAnnals of the New York Academy of Sciences, 928. : 176-181, 2001-
dc.identifier.eissn1749-6632-
dc.relation.journalidJ000778923-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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