Expression of phospholipase C-gamma1 in experimental cholesteatoma using Mongolian gerbils.

Abstract

Phospholipase C (PLC)-gamma1 is known to play a central role in ligand-mediated signal transduction for cell proliferation. The purpose of this study was to elucidate the distribution of PLC-gamma1 in deep meatal skin, retroauricular skin and cholesteatoma matrix of experimental animals. We induced canal ligation cholesteatoma using Mongolian gerbils and investigated the expression of PLC-gamma1 in this model by using Western blot analysis and immunohistochemistry. By Western blot analysis, considerably higher levels of PLC-gamma1 protein were detectable in experimental cholesteatoma. On immunohistochemistry, experimental cholesteatoma showed more intense immunolabeling of PLC-gamma1 protein than deep meatal skin and retroauricular skin. In conclusion, overexpression of PLC-gamma1 may in part contribute to abnormal proliferation and differentiation of experimental cholesteatoma in Mongolian gerbils.