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Bcl-2 blocks cisplatin-induced apoptosis by suppression of ERK-mediated p53 accumulation in B104 cells.
DC Field | Value | Language |
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dc.contributor.author | Park, SA | - |
dc.contributor.author | Park, HJ | - |
dc.contributor.author | Lee, BI | - |
dc.contributor.author | Ahn, YH | - |
dc.contributor.author | Kim, SU | - |
dc.contributor.author | Choi, KS | - |
dc.date.accessioned | 2011-08-25T04:51:57Z | - |
dc.date.available | 2011-08-25T04:51:57Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0169-328X | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/3935 | - |
dc.description.abstract | Bcl-2 has been reported to inhibit neurotoxicity induced by cisplatin. However, neither the mechanism of cisplatin-induced neurotoxicity nor the mechanism by which Bcl-2 confers neuroprotection is clear. In this study, the signaling pathways involved in cisplatin-induced neurotoxicity were examined using a rat neuroblastoma cell line, B104. Treatment of B104 cells with cisplatin induced apoptosis, accompanying the accumulation of p53 and Bax protein. Interestingly, extracellular signal-regulated kinase 1/2 (ERK1/2) activities of MAP kinases were markedly enhanced prior to cisplatin-induced accumulation of p53 and Bax. Inhibition of ERK1/2 activities using PD98059, a selective MEK inhibitor, blocked the apoptotic cell death preventing cisplatin-induced accumulation of p53 and Bax. These results suggest that ERK mediates cisplatin-induced p53 activation to trigger apoptosis in B104 cells. Overexpression of Bcl-2 in B104 cells resulted in the complete resistance to cisplatin-induced apoptosis blocking ERK activation and the subsequent signaling pathway of p53. Our study clearly demonstrates that the action site of Bcl-2 localizes upstream of ERK in cisplatin-induced apoptotic signaling pathway. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antineoplastic Agents | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Cisplatin | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases | - |
dc.subject.MESH | Neuroblastoma | - |
dc.subject.MESH | Proto-Oncogene Proteins | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2 | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.subject.MESH | Tumor Suppressor Protein p53 | - |
dc.subject.MESH | bcl-2-Associated X Protein | - |
dc.title | Bcl-2 blocks cisplatin-induced apoptosis by suppression of ERK-mediated p53 accumulation in B104 cells. | - |
dc.type | Article | - |
dc.identifier.pmid | 11532334 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0169328X01001760 | - |
dc.contributor.affiliatedAuthor | 김, 승업 | - |
dc.contributor.affiliatedAuthor | 최, 경숙 | - |
dc.type.local | Journal Papers | - |
dc.citation.title | Brain research. Molecular brain research | - |
dc.citation.volume | 93 | - |
dc.citation.number | 1 | - |
dc.citation.date | 2001 | - |
dc.citation.startPage | 18 | - |
dc.citation.endPage | 26 | - |
dc.identifier.bibliographicCitation | Brain research. Molecular brain research, 93(1). : 18-26, 2001 | - |
dc.relation.journalid | J00169328X | - |
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