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Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor.

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dc.contributor.authorKim, JY-
dc.contributor.authorYang, MS-
dc.contributor.authorOh, CD-
dc.contributor.authorKim, KT-
dc.contributor.authorHa, MJ-
dc.contributor.authorKang, SS-
dc.contributor.authorChun, JS-
dc.date.accessioned2011-09-19T01:27:59Z-
dc.date.available2011-09-19T01:27:59Z-
dc.date.issued1999-
dc.identifier.issn0264-6021-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/4200-
dc.description.abstractThe signalling pathway leading to an activation of mitogen-activated protein (MAP) kinase subtypes Erk-1 and -2 upon stimulation of muscarinic receptor with carbachol in human neuroblastoma SK-N-BE2(C) cells was investigated. Carbachol activated Erk-1/-2 by stimulating M3 muscarinic receptor, as determined by specific antagonists for individual muscarinic receptors. The activation of Erk-1/-2 by carbachol was blocked by the inhibition or down-regulation of protein kinase C (PKC). Among the multiple PKC isoforms expressed in SK-N-BE2(C) cells, only PKCepsilon was activated by the treatment of carbachol, and selective down-regulation of PKCepsilon was sufficient to block Erk-1/-2 activation. Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Ectopic expression of inhibitory small GTPase Ras, RasN17, blocked the carbachol-induced Raf activation without affecting the activation of PKCepsilon, while the inhibition of PKC blocked the Raf activation. Thus, these results suggest that carbachol-induced activation of PKCepsilon mediates Erk-1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase.-
dc.language.isoen-
dc.subject.MESHCalcium-Calmodulin-Dependent Protein Kinases-
dc.subject.MESHCarbachol-
dc.subject.MESHEnzyme Activation-
dc.subject.MESHHumans-
dc.subject.MESHIsoenzymes-
dc.subject.MESHMitogen-Activated Protein Kinase 1-
dc.subject.MESHMitogen-Activated Protein Kinase 3-
dc.subject.MESHMitogen-Activated Protein Kinases-
dc.subject.MESHProtein Kinase C-
dc.subject.MESHProtein Kinase C-epsilon-
dc.subject.MESHProto-Oncogene Proteins c-raf-
dc.subject.MESHProto-Oncogene Proteins p21(ras)-
dc.subject.MESHReceptor, Muscarinic M3-
dc.subject.MESHReceptors, Muscarinic-
dc.subject.MESHSignal Transduction-
dc.subject.MESHTumor Cells, Cultured-
dc.titleSignalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor.-
dc.typeArticle-
dc.identifier.pmid9882625-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1219962/-
dc.contributor.affiliatedAuthor하, 만준-
dc.type.localJournal Papers-
dc.citation.titleThe Biochemical journal-
dc.citation.volume337-
dc.citation.numberPt2-
dc.citation.date1999-
dc.citation.startPage275-
dc.citation.endPage280-
dc.identifier.bibliographicCitationThe Biochemical journal, 337(Pt2). : 275-280, 1999-
dc.identifier.eissn1470-8728-
dc.relation.journalidJ002646021-
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Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
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