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Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor.
DC Field | Value | Language |
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dc.contributor.author | Kim, JY | - |
dc.contributor.author | Yang, MS | - |
dc.contributor.author | Oh, CD | - |
dc.contributor.author | Kim, KT | - |
dc.contributor.author | Ha, MJ | - |
dc.contributor.author | Kang, SS | - |
dc.contributor.author | Chun, JS | - |
dc.date.accessioned | 2011-09-19T01:27:59Z | - |
dc.date.available | 2011-09-19T01:27:59Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/4200 | - |
dc.description.abstract | The signalling pathway leading to an activation of mitogen-activated protein (MAP) kinase subtypes Erk-1 and -2 upon stimulation of muscarinic receptor with carbachol in human neuroblastoma SK-N-BE2(C) cells was investigated. Carbachol activated Erk-1/-2 by stimulating M3 muscarinic receptor, as determined by specific antagonists for individual muscarinic receptors. The activation of Erk-1/-2 by carbachol was blocked by the inhibition or down-regulation of protein kinase C (PKC). Among the multiple PKC isoforms expressed in SK-N-BE2(C) cells, only PKCepsilon was activated by the treatment of carbachol, and selective down-regulation of PKCepsilon was sufficient to block Erk-1/-2 activation. Carbachol treatment induced activation of the serine/threonine protein kinase Raf, and an inhibition of Raf blocked Erk-1/-2 activation. Ectopic expression of inhibitory small GTPase Ras, RasN17, blocked the carbachol-induced Raf activation without affecting the activation of PKCepsilon, while the inhibition of PKC blocked the Raf activation. Thus, these results suggest that carbachol-induced activation of PKCepsilon mediates Erk-1/-2 activation by a sequential activation of Ras, Raf and MAP kinase kinase. | - |
dc.language.iso | en | - |
dc.subject.MESH | Calcium-Calmodulin-Dependent Protein Kinases | - |
dc.subject.MESH | Carbachol | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Isoenzymes | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1 | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3 | - |
dc.subject.MESH | Mitogen-Activated Protein Kinases | - |
dc.subject.MESH | Protein Kinase C | - |
dc.subject.MESH | Protein Kinase C-epsilon | - |
dc.subject.MESH | Proto-Oncogene Proteins c-raf | - |
dc.subject.MESH | Proto-Oncogene Proteins p21(ras) | - |
dc.subject.MESH | Receptor, Muscarinic M3 | - |
dc.subject.MESH | Receptors, Muscarinic | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Signalling pathway leading to an activation of mitogen-activated protein kinase by stimulating M3 muscarinic receptor. | - |
dc.type | Article | - |
dc.identifier.pmid | 9882625 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1219962/ | - |
dc.contributor.affiliatedAuthor | 하, 만준 | - |
dc.type.local | Journal Papers | - |
dc.citation.title | The Biochemical journal | - |
dc.citation.volume | 337 | - |
dc.citation.number | Pt2 | - |
dc.citation.date | 1999 | - |
dc.citation.startPage | 275 | - |
dc.citation.endPage | 280 | - |
dc.identifier.bibliographicCitation | The Biochemical journal, 337(Pt2). : 275-280, 1999 | - |
dc.identifier.eissn | 1470-8728 | - |
dc.relation.journalid | J002646021 | - |
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