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Molecular role of TGF-beta, secreted from a new type of CD4+ suppressor T cell, NY4.2, in the prevention of autoimmune IDDM in NOD mice.
DC Field | Value | Language |
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dc.contributor.author | Han, HS | - |
dc.contributor.author | Jun, HS | - |
dc.contributor.author | Utsugi, T | - |
dc.contributor.author | Yoon, JW | - |
dc.date.accessioned | 2011-09-27T23:35:57Z | - |
dc.date.available | 2011-09-27T23:35:57Z | - |
dc.date.issued | 1997 | - |
dc.identifier.issn | 0896-8411 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/4252 | - |
dc.description.abstract | A new type of CD4+ T cell clone (NY4.2) isolated from pancreatic islet-infiltrated lymphocytes of acutely diabetic non-obese diabetic (NOD) mice prevents the development of insulin-dependent diabetes mellitus (IDDM) in NOD mice, as well as the recurrence of autoimmune diabetes in syngeneic islet-transplanted NOD mice. It has been demonstrated that the cytokine TGF-beta, secreted from the cells of this clone, is the substance which prevents autoimmune IDDM. This investigation was initiated to determine the molecular role TGF-beta plays in the prevention of autoimmune IDDM by determining its effect on IL-2-induced signal transduction in Con A-activated NOD mouse splenocytes and HT-2 cells. First, we determined whether TGF-beta, secreted from NY4.2 T cells, inhibits IL-2-dependent T cell proliferation in HT-2 cells (IL-2-dependent T cell line) and NOD splenocytes. We found that TGF-beta suppresses IL-2-dependent T cell proliferation. Second, we determined whether TGF-beta inhibits the activation of Janus kinases (JAKs), as well as signal transducers and activators of transcription (STAT) proteins, involved in an IL-2-induced signalling pathway that normally leads to the proliferation of T cells. We found that TGF-beta inhibited tyrosine phosphorylation of JAK1, JAK3, STAT3 and STAT5 in Con A blasts from NOD splenocytes and HT-2 cells. Third, we examined whether TGF-beta inhibits the cooperation between STAT proteins and mitogen-activated protein kinase (MAPK), especially extracellular signal-regulated kinase 2 (ERK2). We found that TGF-beta inhibited the association of STAT3 and STAT5 with ERK2 in Con A blasts from NOD splenocytes and HT-2 cells. On the basis of these observations, we conclude that TGF-beta may interfere with signal transduction via inhibition of the IL-2-induced JAK/STAT pathway and inhibition of the association of STAT proteins with ERK2 in T cells from NOD splenocytes, resulting in the inhibition of IL-2-dependent T cell proliferation. TGF-beta-mediated suppression of T cell activation may be responsible for the prevention of effector T cell-mediated autoimmune IDDM in NOD mice by TGF-beta-producing CD4+ suppressor T cells. | - |
dc.language.iso | en | - |
dc.subject.MESH | Acute-Phase Proteins | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | CD4-Positive T-Lymphocytes | - |
dc.subject.MESH | Calcium-Calmodulin-Dependent Protein Kinases | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | DNA-Binding Proteins | - |
dc.subject.MESH | Diabetes Mellitus, Type 1 | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Interleukin-2 | - |
dc.subject.MESH | Janus Kinase 1 | - |
dc.subject.MESH | Janus Kinase 3 | - |
dc.subject.MESH | Lymphocyte Activation | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred NOD | - |
dc.subject.MESH | Milk Proteins | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Protein-Tyrosine Kinases | - |
dc.subject.MESH | Receptors, Interleukin-2 | - |
dc.subject.MESH | STAT3 Transcription Factor | - |
dc.subject.MESH | STAT5 Transcription Factor | - |
dc.subject.MESH | T-Lymphocytes, Regulatory | - |
dc.subject.MESH | Trans-Activators | - |
dc.subject.MESH | Transforming Growth Factor beta | - |
dc.title | Molecular role of TGF-beta, secreted from a new type of CD4+ suppressor T cell, NY4.2, in the prevention of autoimmune IDDM in NOD mice. | - |
dc.type | Article | - |
dc.identifier.pmid | 9218758 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0896-8411(97)90137-2 | - |
dc.contributor.affiliatedAuthor | 윤, 지원 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1006/jaut.1997.0137 | - |
dc.citation.title | Journal of autoimmunity | - |
dc.citation.volume | 10 | - |
dc.citation.number | 3 | - |
dc.citation.date | 1997 | - |
dc.citation.startPage | 299 | - |
dc.citation.endPage | 307 | - |
dc.identifier.bibliographicCitation | Journal of autoimmunity, 10(3). : 299-307, 1997 | - |
dc.identifier.eissn | 1095-9157 | - |
dc.relation.journalid | J008968411 | - |
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