Aflatoxin Related p53 Codon 249 Point Mutation in Korean Hepatocellular

Abstract

Purpose: P53 point mutation, especially G to T transversion at codon 249, has been found in many cases of the aflatoxin-related hepatocellular carcinoma(HCC). This study was aimed to exam the mutations of the third base at codon 249 of the p53 gene in Korean HCC.

Materials and Methods:Forty two patients with resectable HCC were enrolled. Genomic DNA was prepared by proteinase K digestion and phenol/chloroform extractions. A part of DNA including axon 7 of p53 was amplified by PCR. Restriction fragment polymorphism (RFLP) was analyzed after treatment of Hae III restriction enzyme. And the point mutation were confirmed using the direct sequencing method.

Results: P53 point mutation, G to T transversion at codon 249 was observed in only one tissue specimen(2.4%).

Conclusion: P53 point mutation at codon 249 does not correlate with HCC in Korea.