Allogencic Bone Marrow Transplantation for Severe Aplastic Anemia

Abstract

Allogeneic bone marrow transplantation(BMT) using HLA-identical siblings were carried out in 4 patients with severe aplastic anemia from 1990 to 1995. Patients were conditioned with cyclophosphamide and total lymphoid. irradiation(TLI) or cyclophosphamide, antilymphocyte globulin(ALG) and procarbazine, which provided successful engraftment. in order to prevent mismatched transfusion reaction hemagglutinins in the recipient was removed by plasmapheresis and depletion of erythrocytes from donor marrow could be easily separated using starch sedimentation method. The engraftment was evident with the increase of the peripheral blood counts and increased cellularity in bone marrow biopsies at the fourth week. In 2 cases, mild(Grade I) acute graft-versus-host disease(aGJHD) was developed, but the patients were recovered spontaneously. All of the recipients are now in complete hematologic recovery and in good health more than 48 months, 5 months and 2 months respectively.