Cited 0 times in Scipus Cited Count

UTP and ATPgammaS Induce Mucin Secretion via Ca2+ Dependant Pathways in Human Nasal Epithelial Cells

Other Title
정상 코점막 상피세포에서 UTP와 ASPγS에 의한 Ca2+ 의존성 경로를 통한 점액 분비
Authors
김, 현준  | 최, 재영 | 김, 진영 | 서, 용석 | 이, 정권 | 윤, 주헌
Citation
Journal of the Korean Otolaryngological Society, 46(4). : 302-308, 2003
Journal Title
Journal of the Korean Otolaryngological Society
ISSN
1225-035X
Abstract
BACKGROUND AND OBJECTIVES: Nucleotides such as adenosine triphosphate (ATP) and uridine-5-triphosphate (UTP) play fundamental roles in the early stage of secretion in nasal epithelial cells via P2Y receptor. In the present study, we examined the expression pattern of P2Y subtypes and their functions on Ca2+ influx ([Ca2+]i) in normal human nasal epithelial (NHNE) cells. We also examined the effect of UTP (agonist for P2Y2) and ATPgammaS (agonist for P2Y11) on mucin secretion and mucin gene expression.
MATERIALS AND METHOD: The expression pattern of P2Y receptors and mRNA levels of MUC5AC, MUC5B and MUC8 were examined after treatment with UTP and ATPgammaS by RT-PCR. Mucin was quantitated by immunoblotting assay. We measured the [Ca2+]i in NHNE cells with a double perfusion chamber.
RESULTS: Two uracil-sensitive receptors (P2Y2, P2Y4) and two adenine-sensitive receptors (P2Y1, P2Y11) were expressed in NHNE cells. UTP and ATPgammaS increased [Ca2+]i via caffeine-sensitive pathways, and these two agonists stimulated mucin secretion to a similar magnitude without their gene enhancement. In addition, the mucin stimulatory effects subsided when the intracellular Ca2+ was removed by 2-bis (2-aminophenoxy) ethane-N, N, N', N'-tetraacetic acid-acetoxymethyl ester.
CONCLUSION: Our study showed that P2Y2 and P2Y11 receptors were expressed in NHNE cells and that their agonists, UTP and ATPgammaS, act as secretogogues on mucin secretion via Ca2+-dependent pathways.
Keywords

Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Ajou Authors
김, 현준
Files in This Item:
Journal of the Korean Otolaryngological Society_46(4)_302-308.pdfDownload
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse