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Effect of HGF in Invasion of Hypopharyngeal Squamous Cell Carcinoma Cell Line

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dc.contributor.author김, 철호-
dc.contributor.author문, 성균-
dc.contributor.author배, 준호-
dc.contributor.author이, 재호-
dc.contributor.author최, 은창-
dc.date.accessioned2012-01-13T04:32:33Z-
dc.date.available2012-01-13T04:32:33Z-
dc.date.issued2005-
dc.identifier.issn1225-035X-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/5262-
dc.description.abstractBACKGROUND AND OBJECTIVES: Recent reports revealed that hepatocyte growth factor (HGF) is related to tumor invasion and metastasis. Activation of the urokinase-type plasminogen activator (u-PA)/plasmin proteolytic network and matrix metalloproteinase has been shown to play a key role in tumor invasion and dissemination of various malignancies. So we examined the effect of HGF/c-Met on MMP-2, 9 and u-PA in FaDu cell, a hypopharyngeal squamous cell carcinoma cell line.
MATERIALS AND METHOD: We performed RT-PCR and Western blot in FaDu. Tumor cell invasiveness was assessed by the membrane invasion assay (using Transwell chamber). To examine the role of MMP-2, 9 and the relation between HGF and MMP in the invasion of hypopharyngeal cancer, RT-PCR and zymography were performed in FaDu cells. We tested to confirm the HGF-mediated plasmin activation.
RESULTS: The expressions of c-Met mRNA and protein were detected in the hypopharyngeal cell line while that of HGF was not. HGF markedly enhanced the invasion of cancer cells in a Transwell invasion chamber in a dose-dependent manner (p<0.05). The expression of MMP was detected in hypopharyngeal cancer cells and exogenous HGF slightly enhanced the induction of MMP-2 activity in zymogram analysis. The activity of u-PA was detected in FaDu and HGF (above 10 units/mL) enhanced the activity of u-PA (p<0.05).
CONCLUSION: These results suggest that HGF may play an important role in hypopharyngeal cancer through the activation of u-PA and matrix metalloproteinase.
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dc.formattext/plain-
dc.language.isoko-
dc.titleEffect of HGF in Invasion of Hypopharyngeal Squamous Cell Carcinoma Cell Line-
dc.title.alternative간세포성장인자가 하인두 편평세포암 세포주에서 Matrix Metalloproteinase(MMP)-2, 9과 Urokinase-Type-
dc.typeArticle-
dc.subject.keywordHepatocyte growth factor-
dc.subject.keywordc-Met-
dc.subject.keywordHypopharyngeal cancer-
dc.subject.keywordInvasion-
dc.subject.keywordUrokinase-type-
dc.subject.keywordplasminogen activator-
dc.subject.keywordMatrix metalloptoteinase-
dc.subject.keyword간세포성장인자-
dc.subject.keyword하인두암-
dc.subject.keyword침습-
dc.contributor.affiliatedAuthor김, 철호-
dc.contributor.affiliatedAuthor문, 성균-
dc.contributor.affiliatedAuthor배, 준호-
dc.contributor.affiliatedAuthor이, 재호-
dc.type.localJournal Papers-
dc.citation.titleJournal of the Korean Otolaryngological Society-
dc.citation.volume48-
dc.citation.number6-
dc.citation.date2005-
dc.citation.startPage788-
dc.citation.endPage795-
dc.identifier.bibliographicCitationJournal of the Korean Otolaryngological Society, 48(6). : 788-795, 2005-
dc.relation.journalidJ01225035X-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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