Cited 0 times in
Fibroblast growth factor-21 protects human skeletal muscle myotubes from palmitate-induced insulin resistance by inhibiting stress kinase and NF-κB
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, MS | - |
dc.contributor.author | Choi, SE | - |
dc.contributor.author | Ha, ES | - |
dc.contributor.author | An, SY | - |
dc.contributor.author | Kim, TH | - |
dc.contributor.author | Han, SJ | - |
dc.contributor.author | Kim, HJ | - |
dc.contributor.author | Kim, DJ | - |
dc.contributor.author | Kang, Y | - |
dc.contributor.author | Lee, KW | - |
dc.date.accessioned | 2013-04-22T23:53:00Z | - |
dc.date.available | 2013-04-22T23:53:00Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0026-0495 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/7819 | - |
dc.description.abstract | We investigated the effects of fibroblast growth factor-21 (FGF-21) on palmitate-induced insulin resistance in skeletal muscle myotubes. First, to determine the effect of FGF-21 on palmitate-induced insulin resistance, we measured 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-d-glucose uptake and levels of proteins involved in insulin signaling pathways (IRS-1 and Akt) in human skeletal muscle myotubes (HSMMs) exposed to palmitate for 24h, and compared HSMMs exposed to palmitate and different doses of recombinant FGF-21. Second, to determine the mechanisms underlying the contribution of FGF-21 to palmitate-induced insulin resistance, we compared levels of proteins linked to palmitate-induced insulin resistance (PKC-θ, IKKα/β, JNK, p38, IκBα, and NF-κB) in HSMMs exposed to palmitate and different doses of recombinant FGF-21 for 24h. Palmitate-reduced glucose uptake was restored by FGF-21. Palmitate inhibited phosphorylation of Akt and thereby impaired insulin signaling in HSMMs. FGF-21 prevented palmitate from inhibiting the phosphorylation of Akt. These results indicate that FGF-21 prevented palmitate-induced insulin resistance in HSMMs. Palmitate activated NF-κB in HSMMs, thereby impairing the action of insulin and initiating chronic inflammation. FGF-21 inhibited palmitate-induced NF-κB activation in HSMMs. The results of the present study suggest that FGF-21 prevents palmitate-induced insulin resistance in HSMMs by inhibiting the activation of stress kinase and NF-κB. | - |
dc.language.iso | en | - |
dc.subject.MESH | 4-Chloro-7-nitrobenzofurazan | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Deoxyglucose | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Glucose Transporter Type 1 | - |
dc.subject.MESH | Glucose Transporter Type 4 | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | I-kappa B Kinase | - |
dc.subject.MESH | Insulin | - |
dc.subject.MESH | Insulin Resistance | - |
dc.subject.MESH | MAP Kinase Kinase 4 | - |
dc.subject.MESH | Muscle Fibers, Skeletal | - |
dc.subject.MESH | NF-kappa B p50 Subunit | - |
dc.subject.MESH | Palmitic Acid | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Polymerase Chain Reaction | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.title | Fibroblast growth factor-21 protects human skeletal muscle myotubes from palmitate-induced insulin resistance by inhibiting stress kinase and NF-κB | - |
dc.type | Article | - |
dc.identifier.pmid | 22398021 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0026-0495(12)00040-6 | - |
dc.contributor.affiliatedAuthor | 이, 민석 | - |
dc.contributor.affiliatedAuthor | 안, 소연 | - |
dc.contributor.affiliatedAuthor | 한, 승진 | - |
dc.contributor.affiliatedAuthor | 김, 혜진 | - |
dc.contributor.affiliatedAuthor | 김, 대중 | - |
dc.contributor.affiliatedAuthor | 강, 엽 | - |
dc.contributor.affiliatedAuthor | 이, 관우 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.metabol.2012.01.012 | - |
dc.citation.title | Metabolism: clinical and experimental | - |
dc.citation.volume | 61 | - |
dc.citation.number | 8 | - |
dc.citation.date | 2012 | - |
dc.citation.startPage | 1142 | - |
dc.citation.endPage | 1151 | - |
dc.identifier.bibliographicCitation | Metabolism: clinical and experimental, 61(8). : 1142-1151, 2012 | - |
dc.identifier.eissn | 1532-8600 | - |
dc.relation.journalid | J000260495 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.